Anguis Femineus appeared in 2001 TV series called Kamen Rider Agito. Anguis Femineus (アングィス・フェミネウス Angwisu Femineusu) is a Gorgon-like in appearance and armed with a whip named the Evil-Eyed Whip (邪眼の鞭 Jagan no Muchi). She arrives to aid Masculus on the Over Lord's approval, overpowering Agito and covering their escape. Femineus later battles Agito and G3, forced to retreat after being disarmed by Agito Storm Form. Later, after being denied the Over Lord's aid in healing her, a rage-filled Femineus murdered Sakiko Mikumo to spite him, with the Over Lord telekinetically forcing Femineus to kill herself for breaking the taboo of taking the life of a normal human.
She returned in the Hyper Battle special, only to be destroyed by Agito Flame Form's Double Saber Slash.